Sentence examples for binding were visualized using from inspiring English sources

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Sites of anti-8-OHdG Ab binding were visualized using 3, 3-diamino-benzidine tetrahydrochloride (Vector Labs, Burlingame, CA, USA; SK-4100) as the substrate and hematoxylin as the counterstain.

Sites of binding were visualized using liquid diaminobenzidine (DAB) + substrate + chromogen system (DAKO), counterstained with Mayer's haematoxylin, and photographed by a Nikon OXM1200 digital camera with the Act-1 program.

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EnVision+ System-HRP-Labelled Polymer (DAKO) was used as a secondary antibody and was applied for 1 h at RT. Antibody binding was visualized using Liquid DAB+ Substrate Chromogen System (DAKO).

Antibody binding was visualized using horseradish peroxidase-labeled secondary antibodies and ECL reagent (Amersham).

Pentamer binding was visualized using anti-Pentamer PE and cells were co-stained using CD8 APC (clone SK1, BD).

The membranes were incubated with the appropriate primary and secondary horseradish peroxidase-conjugated antibodies, and the antibody binding was visualized using the ECL detection system (GE Healthcare).

Finally, antibody binding was visualized using DAB as the chromogen and the sections mounted in Mowiol for observation under a Nikon 90i microscope.

Following transfer, blots were thoroughly washed, blocked, probed with primary antibody (Table S4 and S5) and specific binding was visualized using alkaline phosphatase-conjugated secondary antibody and chemiluminescence according to the manufacturer's instructions (Invitrogen).

Specific antibody binding was visualized using a high resolution Leica SP2 AOBS Confocal laser scan microscope (CLSM) (Leica Microsystems Nederland BV, The Netherlands), and images were obtained with Leica confocal software.

Antibody binding was visualized using ECL.

Antibody binding was visualized using the Amersham ECL Plus kit.

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