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Signals from buffer injection and control surface binding were subtracted in all experiments to account for nonspecific binding.
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The values for non-specific binding were subtracted from total binding values (with data expressed in terms of relative fluorescence units, RFU).
Background (mock) binding was subtracted from the signal, which was expressed as a percentage of signals present in untreated cells.
The non-specific binding was subtracted from all other values.
Non-specific binding was subtracted from total binding to obtain specific binding.
Background binding was subtracted from the value obtained for binding to the consensus DNA sequence.
The background was subtracted in both cases.
Background light was subtracted in both cases.
Green channel background (median) was subtracted in all cases.
This background level has been subtracted in all experiments.
First background fluorescence was subtracted in all images.
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