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The blank blood samples for the determination of plasma protein binding were spiked with acetaminophen to obtain a blood concentration of 150 ng/ml.
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Samples were spiked with deuterated pharmaceutical standards as recovery surrogates.
The drugs were spiked with pharmaceutical formulations containing different excipients.
Samples after extraction were spiked with standard concentration and applied.
Samples were spiked with Agilent One-Color Spike-Mix (1 10).
BAL fluid samples were spiked with a positive control to test for spike recovery.
Control specimens were spiked with HEPES buffered saline only.
The tissues were spiked with approximately 10 BCE/g.
All lanes were spiked with a 1 2%% phiX control library.
All lanes were spiked with 1% phiX control library.
The final extracts were spiked with a mixture of analytes.
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