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Interestingly, significant clusters of DGCR8 binding were seen at −1,000/+200 bp from the TSSs and just upstream of the transcription termination regions (TTSs) (false discovery rate [FDR] < 0.01).
However, upon depletion of Otx2, reductions in p300 binding were seen at all loci where inducible p300 binding was observed (e.g., Plekha1 and Myrf loci) but little effect was seen where p300 binding was stably maintained.
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As seen in the peripheral blood, no DARPin binding was seen at later time points.
On all cell types, the greatest binding was seen at the earliest time point (30 minutes) after injection, the amounts decreasing similarly thereafter irrespective of cell type.
However, when the ligands were visualized, no binding was seen at all.
Although CBX8 binding was easily detected at HOXC8 (a known Polycomb target in SEM cells), no change in CBX8 binding was seen at BCL-2.
However, when XAC-X-Texas Red (15) was monitored at the single-cell level, no specific binding was seen at concentrations up to 30 nM.
When XAC-X-TXR (15) was monitored at the single-cell level, no specific binding was seen at concentrations up to 30 nM.
In the absence of L-Trp, zero or negligible binding is observed at 20 60 °C, whereas very weak levels of binding are seen at 10 °C, with an apparent Kd of 280 nM.
The only exception was the results from Lipofectamine-2000™ Lipofectamine-2000™ding whereeen at the completeDNA ratios of ~3, whereas AFM indicated no free DNA (for c-DNA and l-DNA) at the ratio of 1.
A recent example of this process for RNA/protein binding is seen at the single-molecule level, looking at the conformational ensemble of an RNA five-way junction before and after a protein binds (S4 protein binding to a rRNA five-way junction).
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CEO of Professional Science Editing for Scientists @ prosciediting.com