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The reactions were evaluated by ESI-mass analysis and the binding site and modes of binding were revealed by X-ray crystallization analysis.
Positive reaction for positive antibody binding were revealed by addition of TMB (3,3′,5,5′-tetramethylbenzidine 3,3′,5,5′-tetramethylbenzidinete, and read at 650 nm.
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The inhibitor dynamics revealed by the MMDSs suggests that the binding of AHP to BoNTAe is partly entropy-driven.
Antibody binding was revealed by using diaminobenzidine as the chromagen.
After washing, hBAFF binding was revealed by using the HA-peptide specific mAb 12CA5.
Antibody binding was revealed by incubation with anti-mouse (Sigma) or anti-rabbit (Santa Cruz Biotechnology) IgG peroxidase conjugate secondary antibodies.
Antibody binding was revealed by enhanced chemiluminescence.
No nonspecific binding was revealed by the reference flow-cell.
The binding was revealed by using the peroxidase substrate diaminobenzidine (Vector Laboratories) for 1-2 minutes.
Antibody binding was revealed by incubation with horseradish peroxidase-conjugated secondary antibodies (Amersham) and ECL Plus immunoblotting detection (Amersham).
ScFv binding was revealed by 60 min. incubation with NitroBlue Tetrazolium/Bromo-chloro Indoyl Phosphate (NBT/BCIP) substrate.
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