Sentence examples for binding were removed by from inspiring English sources

The brain sections were taken from the hypothalamus (−0.40 and −3.60 mm from the bregma) [47], washed in 0.01 M PBS (Sigma) for GnRH and CRH experiments or in Tris-buffered saline (50 mM Tris, 0.9% NaCl, pH 7.5) for AR experiments; the endogenous peroxidase and nonspecific binding were removed by incubation with 3% H2O2 for 30 min and 0.5% Triton X-100 10 min at room temperature.

The endogenous peroxidase and nonspecific binding were removed by incubation with 3%H2O22 for 10 min and with 10% normal goat serum for 1 h at room temperature.

Excess antibody binding was removed by washing the coverslips with PBS (three times, 5 min).

The supernatant was collected as an unbound fraction, and nonspecific binding was removed by washing 5 times with ice-cold lysis buffer.

Non-specific binding was removed by washes in 2 × SSC at 37°C and single stranded RNA probe digested by incubating the sections for 30 min at 37°C in NTE (500 mM NaCl, 1 mM EDTA, 10 mM Tris pH 8.0) buffer containing 20 μg/ml RNase A (Worthington Biochemical, Reading, UK).

After the binding to TALON metal affinity resins (Takara), non-specific binding proteins were removed by washing with a buffer containing 50 mM Na2HPO4, 0.3 M NaCl, and 30 mM imidazole (pH 7.0).

Proteins binding nonspecifically were removed by sequential washing with a prepared washing solution (4% DMSO in PBS) followed by elution of the proteins in the loading buffer and denaturation by heating (70 °C, 10 min).

Nonspecific binding proteins were removed by washing in cold washing buffer containing 100 mM NaF, 50 mM HEPES (pH 7.5) and 150 mM NaCl for four times followed by one more wash in PBS.

Low-strength, metal-binding proteins were removed by washing with buffer-A containing 50 mM imidazole.

After washing, IgG antibody-binding beads were removed by incubation with IgG-coated iron oxide particles, and the magnetized TentaGel beads were removed using a strong magnet.

Non-binding antibodies were removed by washing four times with PBS containing 1% BSA and 0.01% Triton X-100 and once with PBS.