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Levels of [3H]-resveratrol binding were quantified using a MCID image analysis (St.
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Ligand binding was quantified using an intrinsic tryptophan fluorescence assay and revealed that the detergent-solubilized FLAG-rho1D4-tagged hOR1A1 bound its cognate odorant, dihydrojasmone, with an affinity in the micromolar range.
[11C]Raclopride binding was quantified using BPND values derived from a simplified reference tissue model as previously described (Ikoma et al. 2009).
The primary antibodies binding was quantified using anti- rabbit or mouse IgG HRP secondary antibodies (Amersham) and Super Signal West Pico Chemiluminescent Substrate kit (Pierce).
Antibody binding was quantified using a Typhoon 9400 (GE Healthcare), and goat-anti-mouse IgG-Alexa 488 (Invitrogen) and ImageQuant T software.
[C]AZD2995 binding was quantified using the linear graphical method proposed by Logan et al. [ 26].
The level of binding was quantified using Image J, and results from three separate experiments were fitted in GraghPad Prism (GraphPad Software Inc).
Human PPARγ binding was quantified using the PolarScreenTM PPARγ-Competitor Assay Kit (Invitrogen, Carlsbad, CA), according to the manufacturer's instructions.
Astrocytes were more sparse in the cerebellum than in the cortex and hippocampus, and C-DED binding was quantified using a Simplified Reference Tissue Model (SRTM) in PMOD using the cerebellum as a reference and expressed as non-displaceable binding potential (BPND) for each ROI.
The compounds were docked into 5-LO Xactive site and the binding characteristics were quantified using LUDI.
Chromatin binding proteins were quantified using the Bio-Rad DC protein assay kit (Bio-Rad, Hercules, CA, USA).
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