Binding buffer (BB) used for aptamer binding was prepared by adding baker's yeast tRNA (0.1 mg/mL, Sigma) and BSA (1 mg/mL, Fisher) to the washing buffer to reduce nonspecific binding.
Mutant versions of the MSI1 RNA binding domain were prepared by site-directed mutagenesis using QuikChange (Stratagene).
The tannin-binding mixtures were prepared by mixing 100 mg of PVPP, 1.0 ml of distilled water and 1.0 ml of tannin-containing extracts in a centrifuge tube.
Permanent microscopical preparations were prepared by fixing a small amount of pigment to the Canada balsam or acrylate binding media (Veropal KP 709) dissolved in organic solvents.
Multi-walled carbon nanotube (MWCNT /thionine/gold nanoparticle composites were prepared by binding gold nanoparticles to the surfaces of thionine-coated carbon nanotubes.
In this work, Z-scheme silver chromate-g-C3N4 nanosheets photocatalysts were prepared by binding growth of Ag2CrO4 nanoparticles on the surface of g-C3N4 nanosheets (g-C3N4-N) via a facile precipitation method.
In this work, the stable conjugates of QDs with sulfonated aluminum phthalocyanines (AlPcSs) were prepared by electrostatic binding.
[α-S] UTP-labelled riboprobes for amyloid β (A4) precursor protein (APP) and guanine nucleotide binding protein, alpha inhibiting 2 (genes) genes were prepared by in vitro transcription from corresponding cDNA clones (sense probes were also included for control).
Conjugates 30– 35 were prepared by reacting MET binding peptides 22– 27 with dendrimer 4 (entries 1 6 of Table 1).
The imprinted nanospheres with 67.5 (mg template/of polymer) of binding capacity had better imprinting efficiency than the 50.5% of binding capacity shown by irregularly shaped MIP particles that were prepared by chloroform.
