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In particular, significant relationships between experimental and computed free energies of binding were obtained using Amber PBSA and structures minimized with a distance-dependent dielectric function.
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No binding was obtained using pre-immune serum or Protein G-Sepharose beads only as negative controls.
cprotein binding affinities were obtained using Eq. 6. dsum of the values of the Taft steric parameters of R1and R2. esum of the values of the Taft electronic parameters of R1and R1and
The binding constants were obtained using a 1 1 Langmuir model.
CB1 and CB2 radioligand binding data were obtained using National Institute of Mental Health NIMH Psychoactive Drug Screening Programam (PDSP) resources as described earlier.
Saturable affinity binding curves were obtained using [H]DHA as the radioligand, and propranolol as the unlabelled competitor.
All apparent binding affinities were obtained using the software BIAevaluation v. 2.2.4 (Biacore, GE Healthcare, Chalfont St. Giles, GB).
Transcription factor binding data were obtained using the YEASTRACT programs (Abdulrehman et al. 2011) and gene expression data from published microarray experiments were obtained using the SPELL program (Hibbs et al. 2007).
Serum concentrations of testosterone and sex hormone-binding globulin (SHBG) were obtained using radioimmunoassay.
The study has resulted in the identification of a DNA motif and its derivates strongly supporting HMGA2 binding but the results were obtained using naked DNA instead of chromatin fragments and comprehensive data on its chromatin binding in living cells are missing.
Monoribbed-functionalized iron II) cage complexes, designed for the efficient supramolecular binding with nucleic acids, were obtained using nucleophilic substitution of reactive chlorine atom(s) of the mono- and dichloroclathrochelate bis-α-benzildioximate precursors with primary aliphatic amines, having terminal functionalizing groups, as N-nucleophiles.
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