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Consistent with this, substitution of Phe54 at the tip of the HCDR2 loop of CR6261 with leucine or alanine reduced binding in relation to hydrophobicity, and additive decreases in binding were observed when CR6261 mutants and HA mutants were combined (Fig. 9b).
Similar(59)
However, only a small increase in binding was observed when the Hb concentration increased.
A marked decrease in ATF4 binding was observed when mice are refed 2 h with a control diet after leucine deprivation.
Fig. 2D shows that GST-PBD bound to NPM/B23 while no binding was observed when GST-alone was incubated with NPM/B23.
No binding was observed when only the N-terminal 35 amino acids of Tat were included in the reaction, indicating that these residues are not involved in RSK2 binding (Figure 3D, lane 2).
In addition, both scFvs, moScFv and huScFv, recognized the peptide P1, whereas no binding was observed when peptide P1Q (prolongedged for a single Glu residue at the C-terminus) was used as an antigen in ELISA (Figure 4).
Dose-dependent and saturable binding was observed when increasing concentrations (0 to 1,000 nM) of the recombinant proteins MPL36, LipL40, LipL32, rLIC10494 and rLIC12238 were allowed to individually adhere to a fixed PLG amount (1 µg).
A similar reduction in binding was observed when 500 nM 1 was used as the soluble competitor.
This was true for both GTP- and GDP-loaded Arf1, although more efficient binding was observed when GTP was included in the assay.
No significant binding was observed when sections were preincubated with the aminophenyl mercuric acetate (APMA -activated form of MMP (APMA -activatedtMMP-9 (not shown).
No discernable binding is observed when the drug is titrated against the mitochondrial extract from B2 cells lacking in complex-I.
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