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Assembly of the array, attachment of antibodies and antigen binding were measured using surface plasmon resonance and neutron reflection.
Total binding and non-specific binding were measured using a quartz crystal microbalance with dissipation (QCM-D).
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Expression/Ab binding was measured using flow cytometry.
In all experiments Ab binding was measured using flow cytometry and respective isotype controls were subtracted.
Nucleotide binding was measured using a standard filter-binding assay.
Microtubule binding was measured using the Microtubule Protein Spin-Down Biochem Assay Kit (Cytoskeleton, Inc).
A different kinetic and quantitative assessment of peptide binding was conducted using another flow cytometry assay in which binding was measured using an anti-biotin mouse monoclonal antibody conjugated to AlexaFluor 488 (Invitrogen).
Ligand binding was measured using FITC-labeled TGF-β1 and flow cytometric analysis.
Detection of antibody binding was measured using enhanced chemoluminescence (ECL plus).
GTPγS binding was measured using a filter binding method as described previously (Garcia-Marcos et al., 2010, 2011b).
Following incubation cells were washed three times and binding was measured using FACSCalibur and CellQuest software (BD Biosciences, San Jose, CA, USA).
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