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In the context of this structure, individual ribonucleotides and secondary structural elements that are protected from chemical cleavage or undergo structural transitions upon Rev binding were identified using detailed RNA footprinting experiments.
Genomic sites enriched for H3-K4me3 H3-K27me3me3 binding were identified using ACME (window = 200, thresh = 0.9) and the given p values for each probes from ACME were plotted into graphs using the SAS 9.1 program.
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The region of Myo2 involved in Inp2 binding was identified using mutant variants of Myo2.
Within this construct a RXRα, two Sp1, a CAC-binding protein and a PPARα binding site were identified using the Transfac database via the online-tools TESS and PATCH.
To overcome this issue, several variants with dramatically improved binding rates were identified using a semi-rational strategy, protein ligand binding complex modeling, site-saturation mutagenesis, and HTS for faster binding kinetics.
For a select number of EIN3 targets that are putative transcriptional regulators, DNA-binding motifs were identified using protein binding microarrays (PBM) and the enrichment for these motifs in the promoters of ethylene response genes was determined.
The E2F1 binding sites were identified using the transcription factor search program PROMO [29].
The data in [15] were analyzed using the same pipeline described above for our data; binding sites were identified using an adjusted p-value of 10−4.
6) Putative RNA binding residues were identified using BindN (http://bioinfo.ggc.org/bindn/), RNAbindR (http://bindr.gdcb.iastate.edu/RNABindR/), and KYG (http://yayoi.kansai.jaea.go.jp/qbg/kyg/index.php) [34] [36].
Transcription factor binding sites were identified using the TFSEARCH (http://www.cbrc.jp/research/db/TFSEARCH.html) [75], [76] and TESS (http://www.cbil.upenn.edu/tess/) [77] web servers.
HAMLET binding sites were identified using a synthetic peptide library covering most of the α-actinin-4 sequence (each peptide was 20 amino acids long, with a 5 amino acid overlap).
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