Sentence examples for binding were identified by from inspiring English sources
Median normalization of the log2 values of the ratio of signal to mock (precipitated DNA without antibody) was performed across the three-ChIP-chip arrays followed by a mean centralization to 0. Regions of Pol II binding were identified by the ChIPOTle sliding window method [22]; a window size of 1 Kb was used with a step size of 50 bp.
The amino acids contributing to the antibody binding were identified by mutational analysis [ 22, 23].
Gene ontology categories enriched for Paupar binding were identified by intersecting regulatory regions for known coding genes with Paupar binding sites.
Gene Ontology categories enriched for Dali binding were identified by intersecting regulatory regions for known coding genes with Dali binding sites.
The unique cDNA sequence for the variable domains (VH and VL) containing the complementarity-determining regions (hypervariable domains) responsible for antigen binding were identified by comparison of multiple sequenced clones to the mouse IG set from the ImMunoGeneTics information system for V-QUEry and STandardization (IMGT/V-QUEST) reference directory (28) (Fig. 3 C ).
AnnexinV binding was identified by flow cytometry using an AnnexinV-FITC staining procedure following the manufacturer's instructions (Becton Dickinson).
H4K5Ac binding was identified by ChIP-Seq and was calculated by comparing the control and METH-treated groups after corrections for DNA inputs.
Annexin-V binding was identified by flow cytometry using Annexin-V-FITC staining, following the manufacturer's instructions as previously described (Becton Dickinson, San Jose, CA, USA).
HNF4 and HNF6 binding sites were identified by manual comparison to published consensus binding sequences [36] [39].
To predict which genes are under H-NS control in CAP, putative binding sites were identified by comparative analysis with characterized H-NS binding profiles in related model proteobacteria (see methods).
