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Enthalpic contributions to binding were found by calorimetry to predominate in the differential binding free energy.
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However, since the sites affected by peptide binding were found to be located within a less conserved region of KDM4C, and the phage display also suggested isoform selectivity, the peptide sequences described may be developed into selective inhibitors for individual KDM4 isoforms.
Using the version of the known motif found by MEME and the corresponding unmasked sequences, the positions of the binding sites within these sequences were found by MAST, a companion tool of MEME.
Multilayer binding is found to be regulated by the pH of the solution and the molecular mass and concentration of hyaluronan.
This analysis showed that most residues affected by ataxin-3 binding are found on the solvent-exposed hydrophobic patch of the Ubl domain that surrounds I44 and includes A46, I66, V67, and V70.
The binding constants and binding capacity were calculated, and the nature of binding was found to be noncovalent.
NET-specific binding was found in the locus coeruleus.
The polaron binding energy is found by substitution of Eq. 27 to 26: (26).
The zinc ion binding term was found by both the UniProt keywords and in InterPro [34], which is a database of protein families, domains and functional sites in which identifiable features found in known proteins can be applied to unknown protein sequences.
Based on the specific features and patterns in the per-residue disorder propensity curves calculated by the PONDR ® VLXT tool combined with the analysis of several characteristic sequence-based parameters, short disordered regions capable of undergoing the disorder-to-order transitions on binding can be found by specific predictors.
Consistent with this observation, a larger proportion of genes encoding nucleic acid binding proteins have been found by microarray analysis in HCV-infected cirrhotic livers [ 41].
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