Sentence examples for binding were fit to from inspiring English sources

The time courses of ANO6 current activation and Annexin-V binding were fit to the equation y = A2 + (A1 − A2)/1 + exp[(t − t0)/τ].

Binding isotherms (both direct and competition binding) were fit to a complete competition binding solution for a single site receptor, using Levenberg-Marquardt nonlinear least squares methods, with bound and free fluorescence anisotropy as fit parameters.

We therefore analyzed the data using a more sophisticated approach; the R3 (nonspecific) binding data were fit to the nonspecific finite lattice DNA binding model (eq 3a), while the I1 (cos-specific) binding data were simultaneously fit to the competitive specific/nonspecific finite lattice DNA binding model (eq 3b).

However, binding curves were fit to a nonlinear curve model that considers binding plus linear non-specific binding.

First, the binding data were fit to the nonspecific finite lattice DNA binding model (eq 3a).

Sigmoidal binding curves were fit to the data using weighted least-squares regressions, and the data are presented as fractions of maximal binding based on these fitted curves.

The data points of the binding curves were fit to the equation F = P/ Kd + P) to determine the dissociation constants by Origin 7.5 (Microcal Software Inc ., where F is the fraction of RNA bound, P is the concentration of protein, and Kd is the dissociation constant.

The concentration of HK853 was high enough to ensure minimal depletion after ADP-BODIPY (1) binding, and data were fit to a single-site binding model for determination of the equilibrium dissociation constant, or Kd value.

The resulting binding isotherms (anisotropy versus HOIP concentration) were fit to a 1 1 non-linear binding model (Y=Bmax × X/ Kd+ X)).

Spectra were recorded 20 min after each addition, and the data were fit to a binding equation that accounts for the depletion of ligand upon protein binding.

Sigmoidal competition curves were fit to specific binding data, and the concentration of each steroidal estrogen competitor that inhibited 50% of [3H]E2β binding (IC50) was determined for each mER using GraphPad Prism, using the steps outlined in Supporting Information.