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Specific target linkage and mode of binding were established using co-crystallization and protein mass spectrometry studies.
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Positive and negative control binding regions were established using different primers sets, and data was expressed as percentage of input for each region.
Gating parameters were established using negative controls.
In order to examine the assembly requirements of the TIR1 receptor complex a binding assay was established using SPR.
The contribution of the N protein carboxyl terminus to RNP-HSP72 binding reactions was established using RNP composed of intact N protein or N protein in which the carboxyl terminal 15 kDa was removed by selective proteolysis.
A binding assay was established using P2 rat brain homogenate as described previously (Rapier et al, 1990), with a final protein concentration of 6 mg/ml as determined by a BCA protein assay (Pierce, Rockford, IL, USA).
A salt bridge between Arg5 in TxIA(A10L) and Asp195 of Ac-AChBP was visible in the structure and the importance of this interaction for AChBP binding and nAChR selectivity was established using binding assays, surface plasmon resonance (SPR) and electrophysiological experiments with mutant receptors and conotoxin analogs.
Therefore, the binding capacity of the array must first be established using the tracking oligonucleotide alone, to identify the maximum fluorescein intensity for the slide.
> For the binding immunoassay, the ITP specific assay threshold/baseline was established using mean +3SD and removal of assay values that are outliers.
The route was established using GPS.
Streptavidin-coated donor beads were used in conjunction with glutathione-coated acceptor beads, and the binding was established by monitoring proximity-based luminescent signal.
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