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Saturation constants (Kd and Bmax) for [3H]-AMPA binding were determined with the nonlinear curve-fitting program Ligand software (Biosoft, Cambridge, UK), using a range of unlabelled AMPA [75 10000 nM] added to 50 nM of [3H]-AMPA.
The effect of sequence fidelity on hybridization and aptamer binding were determined with microarrays synthesized with a light-exposure gradient.
Similar(58)
Nonspecific binding was determined with 5 μM -vesamicol.
Non-specific binding was determined with 1 μM unlabelled BN(1 14).
Non-specific binding was determined with 10 µM MLA.
Non-specific binding was determined with 20 µM unlabeled rosiglitazone.
Nonspecific binding was determined with 10 mM GBR 12935 (Sigma).
The biotinylated test antibody MAb 11A8 was added and its binding was determined with peroxidase conjugated streptavidin.
Assessment of peptide binding was determined with sensorgrams, in which the arbitrary response units (RU) were plotted as a function of time.
Ab binding was determined with alkaline-phosphatase conjugated goat anti-mouse H+L (Southern Biotech) for the murine Abs and alkaline-phosphatase conjugated goat anti-human IgG (Caltag) for chAb 18B7.
Non-specific binding was determined with 1 μM THK5117.
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