Sentence examples for binding were determined as from inspiring English sources
Using the same method as described above to obtain the mean values and the standard errors of the mean from three repeated experiments for Δ H°, Ka, n, − TΔ S°, and Δ G° for CaM Fas DD WT binding, the thermodynamic parameters for CaM Fas DD V254N binding were determined as shown in Table 1.
Within the context of a CWR22Pc-R1-AD1 CWR22Pc-R1-AD1 CWR22Pc-R1-AD1ively expresses a strucellally full-length AR containing a H874Y mutation23, the rank order of antagonist backgrounds determined as ARN-509 > Hydroxyflutamide > Enzalutamide > Nilutamide > Bicalutamide (Fig. 3c).
Specific [3H]-DEX binding was determined as the difference between total and nonspecific binding and was expressed as sites/cell.
Specific binding was determined as the difference between the polarization (mP) reading in the absence of competitor to the reading in the presence of a 1000-fold excess of unlabeled reporter peptide.
Serum IgG binding was determined as described above using alkaline phophatase-labeled anti-human IgG antibodies and 4-methylubelliferyl phosphate (1 µg/mL in 0.2 M TRIS, pH 9.5) (Sigma, St . Louis.
Saturable ethylene binding was determined as described by incubation of samples in sealed glass chambers containing either 14C-ethylene (0.1 µL/L) or 14C-ethylene (0.1 µL/L) plus 12C-ethylene (100 µL/L) [10], [22].
The sensitivity defined as two standard deviations above the zero dose binding was determined as 0.6 μg/l, assay range 4 128 μg/l.
The fragments were immobilized at equivalent molar concentrations corresponding to 1 μg/ml FH and antibody binding was determined as described above.
Total binding corresponds to the toxin bound after 100 min of association, and the irreversible binding was determined as the bound toxin that remains after 60 min under dissociation conditions.
