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HEK293 cells were transfected with Flag-Nur77, Nur77 binding were detected with anti-Flag antibody and compared with IgG control antibody (n = 3).
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Specific binding was detected with the Dako REAL EnVision detection system (peroxidase/DAB+, rabbit/mouse) and visualized with diaminobenzidine.
Antibody binding was detected with the enhanced chemiluminescence (ECL) detection system (Amersham Biosciences, Piscataway, NJ).
After incubation with the appropriate secondary antibody for 1 h at room temperature, antibody binding was detected with a HRP-conjugated immunoglobulin G (Santa Cruz) using a chemiluminescent detection system (Westernblotting luminal reagent, Santa Cruz).
Antibody binding was detected with a horseradish peroxidase (HRP -coupled secondary antibody followed by cHRP -coupledence detection (ECL Plusecondaryantibodyacia, Uppsala, Sweden).
After incubation with the appropriate secondary antibody for 1 h at room temperature, antibody binding was detected with an HRP-conjugated immunoglobulin G (Santa Cruz, USA) using a chemiluminescence detection system (Santa Cruz, USA).
Antibody binding was detected with EnVisionTM Dual Link System-HRP DAB kit (K4010, Dako).
No binding was detected with the scrambled oligo (Fig. 3b, top left panel, Supplementary Fig. 5).
In panels (A and B) the Ab binding is detected with AP-conjugated goanti-mouseuse IgG.
In the case of immobilized antibodies anti-Cry1Ac or anti-Cyt1Aa the binding was detected with an antibody anti-RGS-His-HRP.
The binding was detected with an anti-His mAb.
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