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The EC50 titers, representing the concentration for 50% of maximal binding, were calculated by GraphPad Prism.
Tm, enthalpy of unfolding (ΔHu), and change in heat capacity on binding were calculated by fitting the experimental data to equations 9, 24, and 25 from Layton and Hellinga (2010).
For endogenous Myc binding, triplicate sets of ChIP and input DNA were applied on this custom array and genomic sites enriched for c-Myc binding were calculated by ChIP versus input (log2 ratio provided by NimbleGen data output).
Similar(57)
Specific binding was calculated by subtracting nonspecific from total binding at each concentration of radioligand.
Specific binding was calculated by subtracting the non-specific binding (111In-DOTA-belatacept and excess unlabeled belatacept) from the total binding (111In-DOTA-belatacept only).
The degree of binding was calculated by taking the theoretical concentrations calculated from the proteins added to the silica particles originally as 100%.
The specific binding was calculated by the total binding (cpm) - nonspecific binding (cpm).
The specific binding was calculated by subtraction of the non-specific binding from the total binding.
Specific binding was calculated by subtracting the non-specific binding from the total binding.
Non-specific binding was determined by adding an excess of unlabeled protein and specific binding was calculated by subtracting the non-specific binding from the total binding.
Specific binding was calculated by subtracting non-specific binding measured in the presence of a large excess (1.67 µM) of unlabeled insulin.
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