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Endogenous peroxidase activity and nonspecific binding were blocked with 3%H2O22 and nonimmune serum, respectively.
Endogenous peroxidase activity and non-specific binding were blocked with 3%H2O22 and non-immune sera, respectively.
Endogenous peroxidase activity and non-specific binding were blocked with 3% H202 and 50% fetal calf serum (Sigma), respectively, and the sections were incubated overnight with the primary antibodies in a humid chamber at 4°C: mouse D2-40 (M2A antigen, Covance, California, USA) and rabbit anti-ki67 (Abcam ab833).
Endogenous peroxidase and unspecific protein binding were blocked with 0.6% (v/v) H2O2 in TBS (pH 7.6) for 15 min at room temperature and with Ultra V block (Lab Vision, Thermo Fisher Science, Fremont, CA, USA) for 5 min at room temperature, respectively.
Similar(56)
To demonstrate that the nanoworms bind to the same molecular target as Rituximab, binding was blocked with unlabeled Rituximab).
Cells were stained with LIVE/DEAD (Invitrogen) and non-specific binding was blocked with 5 μg/ml anti-CD16/32 (Clone 93, Biolegend).
Nonspecific binding was blocked with bovine serum albumin and normal goat serum, and sections were stained with anti-MMP14 primary antibody (MAB3328; Millipore, Darmstadt, Germany) overnight at 4 °C.
Briefly, remaining free aldehydes were quenched with 50 nm NH4Cl in PBS and unspecific binding was blocked with 1% BSA in PBS.
Sections were permeabilized with PBS/0.2% Tween, and unspecific antibody binding was blocked with 5% horse serum.
Non-specific binding was blocked with 5% Milk-TBS for 1 hour at room temperature.
Non-specific binding was blocked with 5 10% goat serum for 60 minutes.
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