Sentence examples for binding were blocked using from inspiring English sources

Retrieval were performed by proteinase K 2μg/ml (Promega Corporation, Madison, USA) for 5 min at room temperature and nonspecific binding were blocked using 10%% new calf serum and 10%% blocking reagent (Sigma).

Nonspecific binding was blocked using Odyssey blocking solution at room temperature (LI-COR).

This binding was blocked using antibodies to H-2D k) H-2D kt antibuties to H-2K(k).

Nonspecific protein binding was blocked using a 5% milk solution at 4°C overnight.

Non-specific binding was blocked using 5% (v/v) normal sheep serum for 30 min at 37°C.

In brief, unspecific binding was blocked using 1% BSA, 5% normal serum, 0.1% Tween 20 or Triton-X-100 in PBS ( = blocking buffer) for 1 h.

Non-specific binding was blocked using 2% low fat dried milk (marvel) in PBST (blocking buffer) at 37°C for 2 hours.

Non-specific protein binding was blocked using normal goat serum and endogenous peroxidase activity was quenched using 0.3% H2O2 in methanol prior to application of the primary antibody.

Briefly, slides were dried, fixed in 4% paraformaldehyde for 15 minutes and non-specific antibody binding was blocked using 10% normal goat serum.

Non-specific binding was blocked using 3% bovine serum albumin (BSA) in phosphate-buffered saline (PBS) for 1 hour at room temperature (RT) before incubation with ligands.

First, nonspecific binding was blocked using blocking solution (LI-COR).