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Plasma protein binding was assessed using ultrafiltration.
LDL-proteoglycan binding was assessed using surface plasmon resonance.
In addition, specific binding was assessed using autoradiography on ALK5 expressing MDA-MB-231 tumor sections.
AV-45 binding was assessed using an innovative extraction method allowing quantifying uptake in the cortex only.
The total amount of protein binding was assessed using a scintillation counter and results were recorded as counts per minute and compared as a percentage to binding of wildtype Munc18-1 protein.
Nonspecific binding was assessed using mock-transfected cells.
Secondly, striatal D2R binding was assessed using SPECT.
Direct cation binding was assessed using Tb3+ fluorescence analysis.
Nonspecific binding was assessed using an APC-conjugated rat IgG2b antibody as an isotype control.
Nonspecific binding was assessed using a 1000 2000-fold excess of unlabeled ryanodine.
P65 binding was assessed using Cayman's p65 transcription factor assay (Cayman Chemicals, USA).
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