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Secondary structure and substrate binding were analyzed using CD are in good agreement with the in silico predictions.
Both TUNEL and annexin V binding were analyzed using a flow cytometric analysis on a FACSCalibur cytometer (Becton Dickinson, San Josè, CA, USA).
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Chloroquine was conjugated to thiolated gold nanoparticles by using EDC/NHS chemistry and the binding was analyzed using optical density measurement and Fourier transform infrared spectroscopy.
For the binding assay, cells were incubated with increasing concentrations of Rh-labeled EGF, and binding was analyzed using flow cytometry.
Annexin V binding was analyzed using the Annexin V-FITC Detection Kit I (Pharmingen, San Diego, CA, USA) according to the manufacturer's instructions.
Ligand binding data were analyzed using GraphPad Prism 4.03 (GraphPad Software , Inc.
Autoradiograms from ISH and receptor binding studies were analyzed using the optical density readings (grey intensity).
Control sensorgrams were subtracted on line from HS sensorgrams, and the resulting binding curves were analyzed using the Biaeval 3.1 software.
To further analyze the respective GAG binding contributions of the core region and the C-terminal domain of CXCL12, mutations were introduced in both parts of the chemokine (see Fig. 1A) and their binding profiles were analyzed using the SPR assay (Fig. 4).
The binding sensorgrams were analyzed using the BIAevaluation Software.
Ligand binding modes were analyzed using LigandScout 3 software (www.inteligand.com).
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