Sentence examples for binding we obtained a from inspiring English sources

To better characterize the domains in the C-terminal portion of Rb that are responsible for Orc1 binding, we obtained a series of GST fusion proteins carrying the whole Rb C-terminus (fragment AE in Fig. 2D), the A and B pocket domains (fragment AB) or only the C-terminus (fragment SE).

For the DNA-binding and non-DNA-binding dimers, we obtained an R2 value of 0.654 and 0.7427 respectively, concluding that the networks resemble a scale-free topology.

For genome-wide binding of LRH-1, we obtained a BED file from the lab of Tim Osborne (Chong et al., 2012) and annotated distance to TSS with HOMER.

To investigate the binding mode of 1, we obtained a crystal structure of BBOX in complex with 1, N-oxalylglycine (NOG, a structural mimic of 2-oxoglutarate), and NiII (an FeII surrogate) at 1.9 Å resolution (H32 space group; PDB ID: 4CWD).

When we searched the unique MSL2 binding sites for DNA motifs, we obtained a (CAGA n motif that was previously described as a binding site for SMAD3, a transcription factor that translates the TGF-beta receptor response into gene expression regulation (Zawel et al., 1998).

However, when we used higher concentrations of coreBMP2wt we obtained a biphasic binding curve indicating the presence of two different kinds of binding sites.

Second, to eliminate the possibility of GTP as the ligand bound to the putative ATP-binding site in t-CPV, we obtained a 3D reconstruction of SG-CPV at 3.1 Å resolution.

By combining high-resolution binding data for many RNP proteins, we obtained a "multicolored" view of the transcriptome and could readily distinguish overlapping transcripts with different RNP profiles.

We obtained a CCR5 binding density of ∼20 fmol mg−1 protein, which is comparable with other vasoconstrictor peptide receptors including the endothelin ETA receptor (190 ± 23 fmol mg−1) and the thromboxane TP receptor (6 ± 2 fmol mg−1).

For the whole network (adding the DNA and non-DNA binding dimers together), we obtained an R2 value of 0.3935, concluding that the network does not resemble a scale-free topology.

Nonetheless, although we observed subtle changes in backbone NH dynamics upon CO binding, from these we obtain an estimate for the difference in conformational entropy between bound and unbound states of HbI that indicates a significant contribution to HbI cooperativity (from the MD simulations, TΔ S = 1.8 ± 0.6 kcal/mol).