Sentence examples for binding we constructed a from inspiring English sources

In order to determine if SAF-B is recruited to DNA as a result of direct DNA binding, we constructed a GFP-fusion to a truncated SAF-B protein that lacked the N-terminal SAP domain.

To investigate the linker region that may be critical to the Ub binding, we constructed three mutants of AT3-UIM12 with different linkers (Fig. 1C).

To further characterize scFv C1 binding, we constructed and expressed recombinant scFv C1 protein.

To directly test the hypothesis that Msi proteins regulate Jag1 translation via UTR binding, we constructed luciferase reporters for the Jag1 3' UTR and transfected these into 293T cells.

To facilitate identification of potential F12 binding partners we constructed a recombinant virus expressing GST-F12 by rescuing the ΔF12L virus.

To test the specific regulation through the predicted binding site, we constructed a reporter vector consisting of the luciferase coding sequence followed by the 3′-UTR of Smad4.

To be able to predict the location of the PBS (Primer Binding Sites), we constructed a database of tRNAs using tRNAscan-SE (version 1.3.1) (24).

To determine more precisely the actin-binding region, we constructed a PGK-FL5 truncation with an overlapping region of 53 amino acids with PGK-FL4 and in which the first 48 amino acids of PGK-FL3 were missing.

Using the different overrepresented binding patterns, we constructed an interacting transcriptional network.

To determine the region of MTA1 responsible for binding of H3 peptide, we constructed a series of MTA1 deletion mutants.

To identify interface residues that are important for CSP-2B binding to CED-3, we constructed a three-dimensional structural model of the CED-3/CSP-2 complex based on the crystal structure of active caspase-3 22.