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To test for differences between attentional and visuomotor binding, we conducted a unimanual reaching experiment requiring participants to filter out numerous distracting objects.
To ensure specificity of the observed binding, we conducted a control experiment with the Fc-tagged soluble ectodomain of another receptor tyrosine kinase, the platelet-derived growth factor receptor-β (PDGFRβ) subunit, which did not bind Reelin.
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To assess whether the ESS3 sequence alone is sufficient for high-affinity UP1 binding, we conducted calorimetric titrations using a six-nucleotide oligomer that mimics the ESS3b loop sequence.
To assess the effect of USP7 on p53 DNA binding, we conducted electrophoretic mobility shift assays (EMSAs) using a Cy-5 labeled consensus p53 binding sequence as a probe and a version of p53 spanning the core DNA binding and the C-terminal regulatory regions (p5382 393) but lacking the transactivation domain, (Figure 1A).
To identify the residues critical for membrane binding, we conducted co-sedimentation and NMR experiments on a series of F1 mutants.
To further define the sequence elements for AP-2γ binding, we conducted electrophoretic gel mobility shift assays (EMSA) using P-labeled probes corresponding to the potential AP-2γ binding motifs in AP-2γ-ChIP fragments.
As an additional test for calcitriol-regulated Sp-1 binding activity, we conducted a parallel WEMSA analysis, in which the candidate Sp-1-like oligo from CD14 and a canonical Sp-1 oligo were used in parallel.
To determine whether these TSCs are associated with the STAT6 binding site, we conducted a TSS Seq analysis using 27 912 131 TSS tags in STAT6-knockdown BEAS2B cells (Table 2; also see Supplementary Fig. S3E G).
To determine whether deoxygedunin directly binds TrkB, we conducted a ligand binding assay with [3H]deoxygedunin.
Since the number of binding sites used to construct the preliminary profile of CRP binding sites was relatively small (for the reason, see the Results section), in order to minimize possible bias of binding site sampling, we conducted a one-round iteration to obtain a more representative profile of the CRP binding sites.
Towards a practical assessment of the overheads of binding keys to identities, we conducted a quantitative analysis of 17 users' anonymized mailbox extracts to determine which security mechanisms would be most appropriate for their communication patterns.
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