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Ab binding was visualized with StreptABComplex/HRP duet kit and DAB.
Antibody binding was visualized with the enhanced chemiluminescence detection system (Thermo Fischer Scientific).
Antibody binding was visualized with an Immobilon Western Chemiluminescent HRP substrate (Millipore #WBKLS0100).
The binding was visualized with a polyclonal antibody that recognized all human immunoglobulins.
Antibody binding was visualized with 3'-3-diaminobenzidine tetrahydrochloride (DAB; DAB substrate kit for peroxidase, Vector Laboratories).
Antibody binding was visualized with the peroxidase-based anti-rabbit EnVision Kit™ (reference K4003, DAKO) for both antibodies.
Similar(31)
After washing, protein binding was visualized by incubation with HRP-conjugated His antibody or FLAG antibody.
Protein binding was visualized by detecting HRP with the OPD reagent (Pierce, Rockford, IL, USA) that resulted in a colored product.
Specific binding was visualized using alkaline phosphatase avidin together with the respective substrate (Life Technologies).
Antibody binding was visualized by incubating the cells with a secondary antibody; Alexa Fluor-488 conjugated anti-rabbit IgG (1 : 1000, Invitrogen), for 1h in the dark.
E-HRP binding was visualized by the precipitate formed when reacting with 3,3′-diaminobenzidine tetrahydrochloride (DAB) in the presence of urea hydrogen peroxide and CoCl2 for 15 min [SIGMA FAST DAB with metal enhancer tablet set (Co-DAB)].
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