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EnVision+ System-HRP-Labelled Polymer (DAKO) was used as a secondary antibody and was applied for 1 h at RT. Antibody binding was visualized using Liquid DAB+ Substrate Chromogen System (DAKO).

Antibody binding was visualized using horseradish peroxidase-labeled secondary antibodies and ECL reagent (Amersham).

Pentamer binding was visualized using anti-Pentamer PE and cells were co-stained using CD8 APC (clone SK1, BD).

The membranes were incubated with the appropriate primary and secondary horseradish peroxidase-conjugated antibodies, and the antibody binding was visualized using the ECL detection system (GE Healthcare).

Finally, antibody binding was visualized using DAB as the chromogen and the sections mounted in Mowiol for observation under a Nikon 90i microscope.

Following transfer, blots were thoroughly washed, blocked, probed with primary antibody (Table S4 and S5) and specific binding was visualized using alkaline phosphatase-conjugated secondary antibody and chemiluminescence according to the manufacturer's instructions (Invitrogen).

Sites of binding were visualized using liquid diaminobenzidine (DAB) + substrate + chromogen system (DAKO), counterstained with Mayer's haematoxylin, and photographed by a Nikon OXM1200 digital camera with the Act-1 program.

Sites of anti-8-OHdG Ab binding were visualized using 3, 3-diamino-benzidine tetrahydrochloride (Vector Labs, Burlingame, CA, USA; SK-4100) as the substrate and hematoxylin as the counterstain.

Amino acid composition of the binding peptides was visualized using sequence logos [ 39], showing the amino acid enrichment at each position in the binding peptides.

The binding confirmation was visualized using PyMOL.

The antibody binding site was visualized using diaminobenzidine reagent for 5 min. The slides were counterstained with Mayer's hematoxylin.