Sentence examples for binding was visualized by from inspiring English sources

The successful binding was visualized by EM and AFM measurements, and the functioning of the RC was checked by flash photolysis experiments.

After rinsing with 0.1 % PBS-Tween® 20 for 3 × 5 min, antibody binding was visualized by 3,3′-diaminobenzidine using the DAB Peroxidase Substrate Kit Vector Laboratories Ltd.., Peterborough, UK) at room temperature for 5 min.

After washing, protein binding was visualized by incubation with HRP-conjugated His antibody or FLAG antibody.

Antibody binding was visualized by chemiluminescence (Supersignal West Pico Chemiluminescence Kit from Pierce, Bonn).

Antibody binding was visualized by the Dako Envision system (cat #K4011).

Protein binding was visualized by detecting HRP with the OPD reagent (Pierce, Rockford, IL, USA) that resulted in a colored product.

The biotin-free polymeric horseradish peroxidase-linker antibody conjugate system was used in the Bond-maX automatic slide stainer (Vision BioSystems), and antibody-binding was visualized by staining with 3,3-diaminobenzidine (DAB) solution.

Briefly, unspecific binding sites were blocked with 1% blocking reagent for 30 min, successively the labeled probes were bound to anti-digoxigenin Fab-fragments conjugated to alkaline phosphatase for 30 min and, finally, antibody-binding was visualized by membrane incubation in NBT/BCIP solution until the color reaction was completed (30 60 min).

The membranes were then incubated with the respective primary antibodies in Tris-buffered saline milk overnight at 4°C, and specific binding was visualized using species-specific IgG followed by enhanced chemiluminescent detection (ECL kit; Amersham Bioscience, Pittsburg, PA, USA) and exposure to enhanced chemiluminescent X-ray film.

Antibody binding was visualized using ECL.

The impact of this enhanced binding to actin was visualized by changes in podocyte morphology.