Sentence examples for binding was visualised with from inspiring English sources
Antibody binding was visualised with 0.2 mg/ml naphthol-AS-MX-phosphate dissolved in Dimethylforamide, (Sigma), 1 mg/ml Fast Red (Sigma) in 0.1 M Tris buffer (pH 8.2) and 0.25 mg/ml Levamisole (Sigma) for 15 min followed by counterstaining with Mayer's haematoxylin (Dako).
Specific binding was visualised with the peroxidase substrate diaminobenzidine ([DAB] Pierce, Rockford, IL).
Antibody binding was visualised with diaminobenzidin, and then the slides were counterstained with Methylene Green zinc chloride double salt (Sigma).
Slides were washed in PBS and antibody binding was visualised with Zytomed ZytoChem-Plus AP Polymer Kit and Zytomed Permanent AP Red Kit, according to manufacturer's instructions.
The sections were incubated with the avidin biotin peroxidase complex (Vector Laboratories Inc). for 1 h, and antibody binding was visualised with 3,3′-diaminobenzidine tetrahydrochloride.
The sections were incubated with the avidin biotin peroxidase complex (Vector Laboratories Inc). for 1 h, and the antibody binding was visualised with 3,3′-diaminobenzidine tetrahydrochloride.
Primary antibody binding was visualised by incubation with donkey anti-human IgG (NL557; R&D Biosystems), at 5 μg/mL for 2 hours at room temperature.
An antibody binding was visualised by using the secondary antibodies conjugated with horseradish peroxidase and an AEC kit according to the manufacturers' protocols (Dako Corporation, Denmark).
Antibody binding was visualised using diaminobenzidine as the chromagen and slides were counterstained with haematoxylin.
SC-2042; Santa Cruz Biotechnology, Santa Cruz, CA, USA), and their binding was visualised using the streptavidin-biotin complex (Dako, Ely, UK) technique with 3,3'-diaminobenzidine tetrahydrochloride solution (Sigma, Poole, UK).
Following incubation with Envision (Dako Cytomation) (ERBB2) or secondary anti-species antibody, followed by treatment with Streptavidin biotin enzyme complex (StreptAB-Complex, Dako Cytomation), binding was visualised using 3,3′diamino-benzidine tetrahydrochloride (Vector Laboratories).
