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The binding was very weak, with KD values in the micromolar concentration range.
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Interestingly, its binding is very weak; approximately 4 mM Ca2+ was required to saturate the binding.
As expected, the binding is very weak in 7 3 acetone/water, as shown by the small downfield shift of protons c, 0.03 ppm after addition of one equivalent of chloride.
Our free energy calculations agree with this observation, suggesting that for this value of ε binding is very weak: K d = 0.33 M and g cc = − 1.1 k B T. Note that formation of additional bonds in a capsid structure will give rise to substantially more negative binding free energies.
Competition experiments also confirmed that MS1 and MS2 are considerably tighter binders to Mcl-1 than is NoxaA; NoxaA binding to Mcl-1 was very weak and thus difficult to quantify with the competition assay.
There was very weak binding of Hif2α and p300 to HRE-3 site (data not shown).
Syntaxin-immunoreactive bands were pulled down from PC12 cell lysates by Kv2.1-C1 and Kv2.1-C1a peptides (Fig. 1B), but not by the Kv2.1-N or Kv2.1 C2 peptides (the binding of syntaxin to the latter was very weak, if any).
However, the binding of DL-101 to SKW6.4 was very weak suggesting that its epitope, which is on the core protein [ 6], might be masked by glycosaminoglycan on this cell line.
As it is shown in Figure 5A and B, in untreated cells, C/EBP-β binding to the IL-8 promoter was very weak, whereas, within 3 h from PGE2 treatment, the amount of endogenous C/EBP-β that occupied the IL-8 promoter in vivo was consistently higher.
"He was very weak, very weak inside.
Partisanship was very weak.
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