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The affect of salt on the binding of F-σ32 to Tb-core RNAP was confirmed; the binding was very similar with NaCl or KGlu and the binding strength was not greatly weakened, even at 500 mM.
The pattern of MEL18 binding was very similar to that described for other PRC1 components and the H3K27me3 mark, with a peak centered on the first exon of INK4a and very little binding at the ARF promoter region (Fig. 3B and C).
The rate of Annexin-V binding was very similar for 1-6-1_D554-K588 ANO6ANO6.
For the three domains that contain a central aspartate residue (CR3, CR5 and CR8) binding was very similar for a given CR domain for a given set of conditions of ionic strength and pH and showed relatively small change when compared at two ionic strengths and at pH 5.5 and 7.4 (Table 1).
Similar(56)
SAM binding is very similar to other RFM MTases, such as the NS5 protein from dengue virus21 and VP39 protein from vaccinia virus13.
The program reproduced well the position of the triazine ring, but the calculated energies of ligand binding were very similar for different compounds and therefore did not reproduce the observed trends in biological activity.
This situation contrasts with our analyses of the multi-zinc finger insulator proteins Su(Hw) and CTCF which have long binding motifs, where sequence motif matches in the genome are good predictors of binding and where binding is very similar between tissues [28], [62], [74].
The kinetics are investigated in detail below, but we point out here that the modes of binding are very similar to those previously described for WT αS (39).
It should be emphasized that this model for Nod factor binding is very similar to that of the Arabidopsis chitin receptor.
Thus, the role for the secondary binding site in human mincle is analogous to that seen for bovine mincle and these results provide further evidence that the mechanism of binding is very similar in the two proteins.
Concordantly, the TCR residues utilized for binding were very similar in each case (Fig. 2E and F), but again more TCR residues were involved in binding to A2-EAA than A2-ELA.
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