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However, the concentration of the nonspecific binding was very low in all regions, and the equilibrium between the free and the nonspecific ligand was reached relatively quickly, compared with the equilibrium between the other compartments [14].
Non-specific DNA binding was very low in all cases, consistent with the low affinity of Tus for non-specific DNA in 250 mM KCl [25].
We found that for all of the tested genes p53 binding was very low in non-irradiated brains and was substantially increased 2 h after irradiation (Fig. 4E).
When the cells were treated with compactin in the absence of LDL, I-PFO binding was very low, and it rose to a saturating value when increasing amounts of LDL were added.
For both cell types, collagen bead binding was very low and, in contrast to cells that expressed β1 integrin, deoxymannojirimycin enhanced collagen bead binding (Fig. 6D), indicating that the inhibitory effect of deoxymannojirimycin on cell adhesion is dependent on the expression of the β1 integrin.
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None of the other candidate antigens showed this pattern of reactivity with the sera, with the exception of elastin, to which overall levels of IgG binding were very low (data not shown).
Coral cover was very low.
This level of nonspecific binding was very high, so the specific binding signal-to-noise (nonspecific binding) ratio was very low.
EMSA done with equal amount of nuclear extracts (20 μg) and [P]-labeled 1X ABRE-DNA, clearly shows that the level of ABRE-binding-factor was very low and inducible by salinity stress in salt sensitive cultivars (Fig 2B); whereas, very high and constitutive in salt tolerant cultivars (4 to 5 fold higher).
In other words, the level of nonspecific binding of [H]BPA was very low.
Nonspecific binding of the FITC-conjugated IgG2b antibody was very low to non-existent enabling detection of low numbers of CD56-FITC+ cells, whereas PE-conjugated mAbs have slightly higher background staining.
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