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The specificity of antibody binding was verified in tsp-1−/− mice.
The lack of change in binding was verified in an animal study.
For the Cdx-2 and trehalase promoters the HNF4α binding was verified in mouse small intestine epithelium.
A 2-fold increase in β-catenin binding was verified in Lucena cells compared to that in K562 cells after normalization with unspecific binding of protein A. Qualitative analysis of ABCB1 promoter amplification is shown in Figure 2B.
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The effect on miRNA binding was verified by computing the difference in hybridization energy (ΔΔG) caused by that particular SNP (see Materials and methods section).
The specificity of binding was verified through addition of unlabeled probes in competition experiment.
Islet binding was verified by immunostaining the same sections to detect insulin.
Aptamer binding was verified 72 h postsilencing.
Moreover, by co-incubation with excess AMN082 or unlabeled MMPIP, specific binding of [11C]MMPIP with mGlu7 was verified in the brain regions.
The binding of CRT to FasL was verified in this study.
One peptide scored highest in all three algorithms, and the high HLA-A2-binding affinity of this peptide was verified in an HLA stabilization assay.
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