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Redox regulation of the Rap2.4a DNA binding was studied with 2 μg Rap2.4a and 100 pmol F5 in presence of either 5 mM DTT or H2O2 relative to an untreated control.
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Competitive inhibition of bacteria binding was studied by incubating with 1% (w/v) α-D-mannose or purified GP2 (50 μg/ml) in PBS for 2 h at 37°C.
The specificity of the RGD binding was studied on three rats with pre-injection of a blocking dose of cilengitide (18 mg/kg = 30.6 μmol/kg) 10 min prior to the tracer injection [12 15].
The functional consequences of uncoupling PC1/Gα12 binding were studied in apoptosis assays utilizing HEK293 cells with inducible PC1 overexpression.
The nanoscale organization and dynamics of IgE-FcεRI upon antigen binding have been studied with scanning electron microscopy (SEM), localization microscopy, and single-particle tracking (SPT).
The kinetics of the Cl-PMPQ binding onto the preexisting biofilms were studied with UV analyses.
In order to better understand the molecular mechanism of HCIC, the typical ligand of 4-mercaptoethyl-pyridine (MEP) was coupled onto the cellulose matrix, and the binding and departing of IgG were studied with the molecular dynamics simulation.
It has only two spectrin-like repeats and is thought to be Ca++-insensitive with respect to actin binding [ 18]. S. pombe actinin has mainly been studied with regard to contractile ring assembly during cytokinesis.
Transcription factor binding activity was studied by EMSA with human tissue nuclear extracts, including extracts from human brain, followed by EMSA in four different cell line nuclear extracts under different conditions of stimulation or induction.
Competition between Dauda and nonfluorescent fatty acids for binding to KcsA was studied by titration with a fatty acid at a fixed Dauda concentration.
To further investigate the physical interaction between enzyme and substrate, the binding of EndoS and EndoS E235Q) to immobilized polyclonal IgG and IgG1 4 subclasses was studied using slot-binding experiments with immobilized IgG probed with EndoS and EndoS E235Q).
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