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Antibody binding was revealed using fluorescein isothiocyanate (FITC -conjugated secondary antibodies.
Antibody binding was revealed using enhanced chemiluminescence, as per the manufacturer's instructions.
The antibody binding was revealed using peroxidase conjugated goat anti-mouse IgG.
Cerebral blood vessels were detected with an unconjugated polyclonal antibody directed against laminin and binding was revealed using goat anti-rabbit Alexa Fluor® 546 (Invitrogen).
After incubation and a wash step, antibody binding was revealed using a HRP conjugated donkey antibody specific for mouse antibodies for detection of mouse monoclonal IgG2a 8-2.
After incubation and a wash step, antibody binding was revealed using HRP-conjugated goat antibody specific for human Fc for Synagis Mab, a mouse antibody specific for HA-tag (Zymed laboratories) in combination with a HRP-conjugated rabbit antibody specific for mouse antibodies (Dako BV) for detection of Synagis Fab and 101F Fab.
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After they were incubated for 1 h and washed, the binding of the nanopeptamers was revealed using a 1/2000 dilution of the biotinylated HRP conjugate.
Signal was revealed using 3,3' diaminobenzidine (DAB).
Antibody binding sites were revealed using ECL plus the western blotting detection system (GE healthcare).
Further, developments in IMS MS instrumentation has enabled site-specific information about protein conformation and binding interfaces to be revealed using gas-phase HDX labeling [136,137].
Several strong canonical two-part CTCF (motif 1 + 2) binding sites within the PEG13-DMR were revealed using an in silico analysis using the published ChIP-sequence data (data not shown) [ 21, 22].
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