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The binding was retained when the VHL mutation occurred outside of the PIAS4-binding region (R167Q).
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Storage stability was concluded to depend mainly on light exposure while almost all streptavidin binding activity was retained when storing the sensors cold and dark for 8 weeks.
A more recent study claimed that aa-tRNA binding to EF-Tu was retained when Cys81 is replaced by Ala.
Even following CatG cleavage, peptide binding was retained by pre-loaded, soluble recombinant HLA-DR.
The high-affinity of well-characterized of GAL4 DNA-binding has previously been shown to be retained when placed in the context of a heterologous fusion partner or used to enhance gene delivery through conjugation of ligand and other cationic polymers [ 1, 2, 17].
Factors were retained when eigenvalue was greater than 1 [ 42].
These mixed patterns were retained when individual colonies were tested.
Thus specificity of binding is retained after labeling, and the affinity is almost unperturbed.
Most problematic is the observation that the activity due to tight pterin binding is also retained when the enzyme is incubated overnight with an excess of BH4.
In the context of mitogenic stimulation, we find that P38i alone can block H3S28ph and chromatin dissociation of the PRC1 protein BMI1, whereas BMI1/chromatin binding is largely retained when ERK signaling is interrupted (MEKi), without affecting H3S28ph (Additional file 1: Figure S1B).
Most (>99%) of the total gp120-binding rAH was retained in the insoluble pellet fraction.
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