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Apparently cell surface binding was required to detach the viral DNA from the core membrane and de-condense it, such that it could be released as a whole into the cytoplasm for subsequent cytoplasmic DNA-replication.
To overcome this, the basal rate of Gt binding was required to be significantly faster.
Similar(57)
The observation of cellular colocalization and physical binding are required to demonstrate a cellular interaction between two components.
Repressor function in EcBirA is in association with bio-5'-AMP (co-repressor) and hence very tight binding is required to perform this function (26).
TFIIIC binding is required to recruit TFIIIB at most Pol III promoters.
CTCF binding is required to maintain the maternal IC1 hypomethylated during early embryogenesis (9).
We therefore conclude that RNA-stimulated ATP binding is required to induce signaling by RIG-I.
As in the case of PP5, substrate binding is required to stimulate the activity of DUSP1, DUSP2, DUSP6 and DUSP9.
KrasG12D expression is necessary but not sufficient to initiate neoplasia; GTP binding is required to activate KrasG12D (Huang et al., 2014).
As a result, while the stoichiometry of CLC binding to soluble tubulin is approximately 1 mol per mol tubulin [ 31] much less CLC with very little binding is required to inhibit the dynamic instability of microtubules [ 32].
In their work, Nielsen et al. also showed that mutating the Leu3p binding site reduced the transcription level of BAP2 on SC medium and concluded that Leup3 binding is required to obtain full BAP2 promoter activity.
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