Sentence examples for binding was removed by from inspiring English sources

Excess antibody binding was removed by washing the coverslips with PBS (three times, 5 min).

The supernatant was collected as an unbound fraction, and nonspecific binding was removed by washing 5 times with ice-cold lysis buffer.

Non-specific binding was removed by washes in 2 × SSC at 37°C and single stranded RNA probe digested by incubating the sections for 30 min at 37°C in NTE (500 mM NaCl, 1 mM EDTA, 10 mM Tris pH 8.0) buffer containing 20 μg/ml RNase A (Worthington Biochemical, Reading, UK).

The brain sections were taken from the hypothalamus (−0.40 and −3.60 mm from the bregma) [47], washed in 0.01 M PBS (Sigma) for GnRH and CRH experiments or in Tris-buffered saline (50 mM Tris, 0.9% NaCl, pH 7.5) for AR experiments; the endogenous peroxidase and nonspecific binding were removed by incubation with 3% H2O2 for 30 min and 0.5% Triton X-100 10 min at room temperature.

The endogenous peroxidase and nonspecific binding were removed by incubation with 3%H2O22 for 10 min and with 10% normal goat serum for 1 h at room temperature.

As shown in Fig. 4A, at each time interval in the course of the incubation, HIV-1 p24 binding was removed from the cells by EDTA to an equal extent at 37°C and 4°C.

After binding, unbound FAM-Aβ42 was removed by three washes with cold PBS.

Then, PEG grafting was performed by covalent binding, and unbound PEG was removed by rinsing with PBS in an ultrasonic bath as explained above.

28 Prior to docking simulation, the influenza NP418 epitope, which was complexed in the binding groove of HLA-B*3501 HLA-B*3501ed by using AutoDockTools.

Then, the proteins were recovered from the GST-tag by thrombin cleavage and thrombin was removed by binding to benzamidine-Sepharose.

Free ubiquitin was removed by binding the modified PCNA to a 1 ml HiTrap Q column and washing extensively with buffer A+200 mM NaCl.