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When, however, the M2 and C-term regions were both deleted, then PABPC1 binding was reduced, suggesting that these regions work in concert to bind PABPC1.
Pimonidazole binding was reduced after 3 h p.i. by 30% which is similar to the 21% reduction of [18F]FAZA.
In higher brain centres, [3H]NBTI binding was reduced in the paraventricular thalamic nucleus of both heterozygous and homozygous mice, whereas [3H]DPCPX binding was reduced in the hippocampus and lateral hypothalamus of heterozygotes.
When VASC-1 was co-cultured with SCP2 knockdown rat vascular endothelial cells, VASC-1 binding was reduced significantly.
In the in vitro autoradiography experiment, 111In-DOTA-belatacept bound to human carotid plaques and the radiotracer binding was reduced under blockade conditions with excess of unlabeled belatacept (Fig. 5a, corresponding plaque samples to Fig. 4).
Furthermore, both AP-2α and HDAC binding was reduced in the AP-2α downregulated cells.
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Consequently, in the presence of the vinculin tail, FAK-paxillin binding is reduced, resulting in a reduced activation of ERK and PAK (18, 57).
The mutation is located on a part of the protein that normally binds with the LDL receptor, and binding is reduced as a result of the mutation.
The ChIP assays revealed that endogenous and overexpressed Runx2 binds to mTOR promoter and that this binding is reduced by Runx2 knockdown.
Given that SP1 and SP3 compete for binding sites, it is possible that reduced binding of endogenous SP1 to the methylated C and T alleles combined with the increased SP3/SP1 ratio may increase the number of cells in which SP3 can bind over rs143383, even though the affinity of SP3 binding is reduced by methylation.
Compared to docking, the free-energy barrier for binding is reduced when the single α-helix is allowed to fold upon binding, but only marginally.
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