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An apparent steady state of binding was reached after 6 h at 15 °C with lamprey insulin; 4 h was required for hagfish insulin, while human insulin required incubation for 2.5 h.
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Measurements taken after 228 h (not shown) revealed that 2.0 mM was bound, indicating that the maximum calcium binding capacity was reached after 156 h.
As kinetic binding experiments revealed that approximate equilibrium was reached after a 3 h incubation period with scFv (6 C and D), the incubation time for binding studies was adjusted to this incubation time.
In vitro binding kinetics of the PEGylated analogue was slower since steady-state condition was reached after 4 h.
Convergence was reached after 3 iterations.
Convergence was reached after two iterations.
Full saturation was reached after five months and was mainly reached by natural hydration.
This point was reached after 22 interviews.
Stationarity was reached after 40,000 generations.
Data saturation was reached after 12 interviews.
A consensus was reached after discussion.
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