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Ligand binding was quantified using an intrinsic tryptophan fluorescence assay and revealed that the detergent-solubilized FLAG-rho1D4-tagged hOR1A1 bound its cognate odorant, dihydrojasmone, with an affinity in the micromolar range.
[11C]Raclopride binding was quantified using BPND values derived from a simplified reference tissue model as previously described (Ikoma et al. 2009).
The primary antibodies binding was quantified using anti- rabbit or mouse IgG HRP secondary antibodies (Amersham) and Super Signal West Pico Chemiluminescent Substrate kit (Pierce).
Antibody binding was quantified using a Typhoon 9400 (GE Healthcare), and goat-anti-mouse IgG-Alexa 488 (Invitrogen) and ImageQuant T software.
[C]AZD2995 binding was quantified using the linear graphical method proposed by Logan et al. [ 26].
Human PPARγ binding was quantified using the PolarScreenTM PPARγ-Competitor Assay Kit (Invitrogen, Carlsbad, CA), according to the manufacturer's instructions.
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Levels of [3H]-resveratrol binding were quantified using a MCID image analysis (St.
The overrepresentation of binding sites was quantified using z-scores (Table 1).
[11C](R -PK11195 binding to TSPO was quantified using kinetic compaR -PK11195lyses with the metabindingcorrectod arTSPOal plasma curve as input function.
The SERT nondisplaceable binding potential (BPND) (40) was quantified using the multilinear reference tissue model (41).
The binding interaction between ITP and MA3706 was quantified using a Microcal VP Titration Calorimeter.
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