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Fusion protein binding was evaluated by FITC-anti-His-mAb labeling, and hapten binding was quantified from Cy5-Him.
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[11C]Raclopride binding was quantified using BPND values derived from a simplified reference tissue model as previously described (Ikoma et al. 2009).
The level of binding was quantified using Image J, and results from three separate experiments were fitted in GraghPad Prism (GraphPad Software Inc).
Autoradiogaphic I-receptor binding was quantified by using ImageJ measurement and specific binding was calculated by subtracting nonspecific binding from the total binding for each area.
The cells were washed, incubated with the second dye-labelled probe for 30 min, and the binding was quantified by flow cytometry.
The plates were visualized at 450 nm and binding was quantified by color using a Spectra Max 250 plate reader.
Radioligand binding was quantified by kidney-to-blood ratio (KBR), standard uptake value (SUV), and distribution volume (DV).
ATP-γ35S binding was quantified as previously described [94].
After four washings, PLG binding was quantified by specific antibodies as described above.
Arrestin-1 binding was quantified by liquid scintillation counting.
(B ) Fc binding was quantified by analyzing the percent area of GFP co-stained with Fc.
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