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TSPO binding was quantified as total distribution volume of [11C]PBR28 in grey matter, controlled for polymorphisms in the TSPO gene that affect binding affinity.
ATP-γ35S binding was quantified as previously described [94].
Each PCR was run in triplicate and protein binding was quantified as a percentage of total input material.
The mitochondrial DNA content was quantified by utilizing a fluorometric assay where an increase in SYBr Green (Invitrogen Molecular Probes) fluorescence because of higher DNA binding was quantified as a measure of the amount of DNA content.
Each PCR was run in triplicate and protein binding was quantified as a percentage of total input material, calculated as described by Wierzbicki (protocol available at: http://www.mcdb.lsa.umich.edu/labs/wierzbicki/protocols.php).php
Similar(55)
After four washings, PLG binding was quantified by specific antibodies as described above.
[11C]Raclopride binding was quantified using BPND values derived from a simplified reference tissue model as previously described (Ikoma et al. 2009).
The cells were washed, incubated with the second dye-labelled probe for 30 min, and the binding was quantified by flow cytometry.
Arrestin-1 binding was quantified by liquid scintillation counting.
Proteins were quantified as above.
To compare their binding preference, we used a receptor binding
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