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Albumin binding was proven by agarose gel electrophoresis.
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The specificity of the binding was proved by adding free testosterone (200 nM) into the measuring solution and incubating for approximately 1 h to block the ligand-binding sites of the proteins.
The specificity of the binding was proved by means of displacement with cold radioligands: more than 92% of the specific [In]-DOTA-dLVT binding in OTR-positive cells was displaced by 5 min of preincubation with 100 μ M and 1 m M nonradioactive OT or LVT.
Specific binding of recombinant scFv was proven by flow cytometry.
The cooperativity between the bisquinoline and pyrrole oligoamide moieties for good binding affinity to G-quadruplex was proven by synthesizing 2 and 3 lacking a quinoline ring and pyrrole amide, respectively, and both show much reduce affinity to G-quadruplex.
Dopaminergic activity via binding to dopamine-2 (DA-2) receptors [17] was proven by several experimental approaches: 1. Radioreceptorassays The D2 receptor is nowadays available as recombinant protein.
Therefore, the binding free energies were positively correlated with the bioaccumulation of PCBs, which was proven by molecular modeling.
The theorem was proven by Boris Trakhtenbrot (1950).
CFSGS was proven by biopsy.
This was proven by stereotactic biopsy.
This was proven by the Mythbusters.[2].
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binding was enhanced by
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