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Non-specific rhodopsin binding was probed using non-immune mouse IgG.
Non-specific protein binding was probed using either non-immune mouse IgG or 1D4 antibody treated with its epitope blocking peptide.
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To define how cap behavior is coordinated at the four binding pockets of the enzyme, the PAPS dependence of the open or closed status of the acceptor binding sites was probed using a large acceptor [4-hydroxytamoxifen (TAM)].
The binding of LBP to LPS was probed using a polyclonal anti-LBP Ab (Abcam).
The interaction of CO2 with the materials under irradiation was probed using in-situ FTIR to interrogate the role of oxygen vacancies in CO2 binding and reaction.
The mRNA binding abilities of M. tuberculosis ThyA and ThyX were probed using a gel-shift assay (Fig. 3).
Anion-binding studies in solution: Anion binding was probed by using NMR, luminescence and electrochemical methods in assorted solvents as dictated by various factors.
The binding properties of brazilin and Zn2 + were first probed using Fourier transform infrared (FTIR) spectroscopy and isothermal titration calorimetry (ITC) assays.
The specificity of NF-κB binding was proven using excess, unlabelled NF-κB probe that competed successfully for NF-κB binding, whereas an unrelated competitor (activator protein-1 oligonucleotide) did not (data not shown).
The SbMYB44 protein showed increased binding with increasing concentration of the probe, the maximum binding was observed using 80 ng probe with all the cis-elements (Fig. 5A and B).
Wells were probed with a TF2-horseradish peroxidase conjugate, and binding was revealed using o-phenylenediamine dihydrochlorides.
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