Sentence examples for binding was prevented using from inspiring English sources
Avidin biotin non-specific binding was prevented using a blocking kit according to the manufacturer's protocol (Vector Laboratories, Inc., Burlingame, CA, USA).
Unspecific binding was prevented using 5% non fat dry milk (w/v) in TBS-T0.1% buffer (50 mM Tris, 150 mM NaCl, pH 7.4, 0.1% Tween-20) for 1 h at room temperature.
Non-specific binding of the avidin/biotin system was prevented using the avidin/biotin blocking kit (Vector Laboratories, Burlingame, USA).
This increase in binding was prevented by inactivation of HMGA1a in the nuclear extracts using an antibody against it (Figures. 2A and 2B).
Non-specific binding was prevented by blocking with normal goat serum (1 10) for 10 min. Immunostaining of survivin was performed using a rabbit anti-human survivin polyclonal antibody.
Such problems can be prevented through staining whole blood rather than a cell suspension, or through the use of a blocking reagent, by which Fc receptors are kept saturated and non-specific binding is prevented.
Cells were stained for 30 min at 4°C and non-specific binding prevented using anti-mouse CD16/32 mAb (Mouse BD Fc Block™; 2.4G2).
The possible binding of antigen present in plasma to erythrocytes after blood sample collection was prevented by the use of EDTA as anti-coagulant.
The contribution of c-Jun binding to GR binding was examined using A-FOS, a dominant negative protein that heterodimerizes with c-Jun and prevents DNA binding [ 22, 33].
