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A photoreactive initiator for surface-initiated atom-transfer radical polymerization (SI-ATRP) was attached to a silicon oxide surface and a polymer brush resistant to non-specific binding was prepared from it.
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Extracts for RNA binding were prepared from purified amaranth chloroplasts as described [ 26].
For examining the level of ncRNAs under silencing of the core RNA binding proteins, RNA was prepared from untreated cells and 3 days after the induction of silencing, as previously described [ 48, 49, 77, 78].
For these experiments, a conjugate was prepared from non-binding M1234-cys-anxA5 under identical reaction conditions and compared with binding anxA5-DTPA 6 in terms of their capacity to displace anxA5-FITC.
Interestingly, although the chromatin was prepared from heart, TRα1 binding also occurred on the TREs of RC3 and hairless genes that have not been reported to be expressed in this tissue.
The effector plasmid pABM2(IFN-β1) was prepared from the plasmid pABM2 with GAL4-binding sites and coding for firefly luciferase, a kind gift from Dr. Mark S. Ptashne.
[3H]PhTX-56 was prepared from a diiodo-precursor with high specific radioactivity, providing the first radiolabeled ligand binding to the pore-forming part of AMPA receptors.
Deoxyartemisinin was prepared from artemisinin by hydrogenation.
Actin was prepared from chicken breast [22].
Chromatin was prepared from approximately 150 discs.
RNA was prepared from the frozen lymphocytes.
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