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Exact(14)
Competition for OsSND2 binding was performed with unlabelled P9 fragments containing SNBE1 site at 50×, 100× and 200× the amount of labeled probe.
Blocking of unspecific binding was performed with FCS/TRIS 20%.
Blocking of unspecific binding was performed with 2% BSA/PBS.
Differential analysis of transcription factor binding was performed with sTRAP software (http://epicenter.immunbio.mpg.de/cgi-bin/chromos/sTrap.cgi).
GC-background (GCbg) correction for non-specific probe binding was performed with APT, using feature signals derived from 33,894 GC-background probes on the array.
Blocking of non-specific binding was performed with 1% (w/v) bovine serum albumin and 10% (v/v) goat serum (DAKO) in PBS for 1 h at RT.
Similar(46)
Three replicate bindings were performed with two distinct preparations for all the bacterial surface molecules tested with very similar results.
Blocking to prevent unspecific binding was performed overnight with 5% milk powder in 0.1% Tween 20 in PBS containing 0.02% NaN2 at 4°C.
Blocking of non-specific antibody binding was performed by incubation with normal goat serum at 37 °C.
Blockade of nonspecific binding was performed by incubation with PBS/bovine serum albumin (Fraction V, Thermo Fisher Scientific, Geel, Belgium).
Blocking against non-specific binding was performed for 60 min with 0.5 % goat serum dissolved in PBST, and the cells were again washed three times with PBST.
More suggestions(15)
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